Restriction endonuclease Fok1, C terminal | |||||||||
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Identifiers | |||||||||
Symbol | Endonuc-Fok1_C | ||||||||
Pfam | PF09254 | ||||||||
Pfam clan | CL0415 | ||||||||
InterPro | IPR015334 | ||||||||
SCOP2 | 2fok / SCOPe / SUPFAM | ||||||||
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The restriction endonuclease Fok1, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non sequence-specific DNA cleavage domain at the C-terminal.[2] Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3' recognition site, the DNA cleavage domain is activated and cleaves the DNA at two locations, regardless of the nucleotide sequence at the cut site. The DNA is cut 9 nucleotides downstream of the motif on the forward strand, and 13 nucleotides downstream of the motif on the reverse strand,[3] producing two sticky ends with 4-bp overhangs.
Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
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