Gel permeation chromatography

Gel permeation chromatography (GPC)^[1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers. As a technique, SEC was first developed in 1955 by Lathe and Ruthven.^[2] The term gel permeation chromatography can be traced back to J.C. Moore of the Dow Chemical Company who investigated the technique in 1964.^[3] The proprietary column technology was licensed to Waters Corporation, who subsequently commercialized this technology in 1964.^[4] GPC systems and consumables are now also available from a number of manufacturers. It is often necessary to separate polymers, both to analyze them as well as to purify the desired product.

When characterizing polymers, it is important to consider their size distribution and dispersity (Đ) as well their molecular weight. Polymers can be characterized by a variety of definitions for molecular weight including the number average molecular weight (M_n), the weight average molecular weight (M_w) (see molar mass distribution), the size average molecular weight (M_z), or the viscosity molecular weight (M_v). GPC allows for the determination of Đ as well as M_v and, based on other data, the M_n, M_w, and M_z can be determined.