Antisense RNA (asRNA), also referred to as antisense transcript,[1] natural antisense transcript (NAT)[2][3][4] or antisense oligonucleotide,[5] is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein. The asRNAs (which occur naturally) have been found in both prokaryotes and eukaryotes,[1] and can be classified into short (<200 nucleotides) and long (>200 nucleotides) non-coding RNAs (ncRNAs).[4] The primary function of asRNA is regulating gene expression. asRNAs may also be produced synthetically and have found wide spread use as research tools for gene knockdown. They may also have therapeutic applications.[6][1][4]
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