Assisi is a town and commune in Italy.
Assisi may also refer to:
- Assisi Catholic College, on the Gold Coast in Upper Coomera, Queensland, Australia
- Assisi Hospice, Singapore
- Assisi Animal Sanctuary, Conlig, County Down, Northern Ireland
- Assisi embroidery, a form of counted-thread embroidery
- Assisi (meteorite), which fell to earth in 1886 at Umbria, Italy
- Sergio Assisi (born 1972), Italian actor
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