Basilissa (Ancient Greek: Βασίλισσα) is a feminine form of the Greek title basileus ("king" or "emperor").
Basilissa may also refer to:
- Basilinna or Basilissa, the ritual queen who was wed to Dionysus during the Anthesteria in ancient Athens
- Basilissa (gastropod), a genus in the family Seguenziidae
- Basilissa (name)
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