DNA ligase

DNA ligase
Artist's conception of DNA ligase repairing chromosomal damage
Identifiers
EC no.	6.5.1.1
CAS no.	9015-85-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

ligase I, DNA, ATP-dependent
Identifiers
Symbol	LIG1
NCBI gene	3978
HGNC	6598
OMIM	126391
RefSeq	NM_000234
UniProt	P18858
Other data
Locus	Chr. 19 [1]
Search for Structures Swiss-model Domains InterPro

DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template,^[1] with DNA ligase creating the final phosphodiester bond to fully repair the DNA.

DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.