Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics.[1] Live-cell imaging was pioneered in the first decade of the 21st century. One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg.[2] Since then, several microscopy methods have been developed to study living cells in greater detail with less effort. A newer type of imaging using quantum dots have been used, as they are shown to be more stable.[3] The development of holotomographic microscopy has disregarded phototoxicity and other staining-derived disadvantages by implementing digital staining based on cells’ refractive index.[4][5]
- ^ Baker M (August 2010). "Cellular imaging: Taking a long, hard look". Nature. 466 (7310): 1137–1140. Bibcode:2010Natur.466.1137B. doi:10.1038/4661137a. PMID 20740018. S2CID 205056946.
- ^ Landecker H (October 2009). "Seeing things: from microcinematography to live cell imaging". Nature Methods. 6 (10): 707–709. doi:10.1038/nmeth1009-707. PMID 19953685. S2CID 6521488.
- ^ Jaiswal JK, Goldman ER, Mattoussi H, Simon SM (October 2004). "Use of quantum dots for live cell imaging". Nature Methods. 1 (1): 73–78. doi:10.1038/nmeth1004-73. PMID 16138413. S2CID 13339279.
- ^ Pollaro, L.; Equis, S.; Dalla Piazza, B.; Cotte, Y. (2016). "Stain-free 3D Nanoscopy of Living Cells". Optik & Photonik. 11: 38–42. doi:10.1002/opph.201600008.
- ^ Pollaro, L.; Dalla Piazza, B.; Cotte, Y. (2015). "Digital Staining: Microscopy of Live Cells Without Invasive Chemicals" (PDF). Microscopy Today. 23 (4): 12–17. doi:10.1017/S1551929515000590. S2CID 135982205.
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