The Ohio Valley is the valley which surrounds the Ohio River.
Ohio Valley may also refer to:
- Ohio Valley Conference, NCAA Division I athletic conference
- Ohio Valley Hospital, not-for-profit hospital in Kennedy Township, Pennsylvania
- Ohio Valley Mall, indoor shopping mall in St. Clairsville, Ohio
- Ohio Valley Redcoats, former minor league baseball team
- Ohio Valley–style pizza, style of pizza popular in Ohio and West Virginia
- Ohio Valley University, former private Christian college in Vienna, West Virginia
- Ohio Valley Wrestling, American professional wrestling promotion
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