Reverse Transcription Loop-mediated Isothermal Amplification

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA. It is used to diagnose infectious disease caused by RNA viruses.^[1]

It combines LAMP^[2] DNA-detection with reverse transcription, making cDNA from RNA before running the reaction.^[3] RT-LAMP does not require thermal cycles (unlike PCR) and is performed at a constant temperature between 60 and 65 °C.

RT-LAMP is used in the detection of RNA viruses (groups II, IV, and V on the Baltimore Virus Classification system), such as the SARS-CoV-2 virus^[4] and the Ebola virus.^[5]

^ Mori Y, Notomi T (2009). "Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases". J. Infect. Chemother. 15 (2): 62–9. doi:10.1007/s10156-009-0669-9. PMC 7087713. PMID 19396514.
^ Notomi, Tsugunori; Okayama, Hiroto; Masubuchi, Harumi; Yonekawa, Toshihiro; Watanabe, Keiko; Amino, Nobuyuki; Hase, Tetsu (2000-06-15). "Loop-mediated isothermal amplification of DNA". Nucleic Acids Research. 28 (12): e63. doi:10.1093/nar/28.12.e63. ISSN 0305-1048. PMC 102748. PMID 10871386.
^ Fu S, Qu G, Guo S, Ma L, Zhang N, Zhang S, Gao S, Shen Z (2011). "Applications of loop-mediated isothermal DNA amplification". Appl. Biochem. Biotechnol. 163 (7): 845–50. doi:10.1007/s12010-010-9088-8. PMID 20844984. S2CID 45682156.
^ Habibzadeh, Parham; Mofatteh, Mohammad; Silawi, Mohammad; Ghavami, Saeid; Faghihi, Mohammad Ali (2021-02-17). "Molecular diagnostic assays for COVID-19: an overview". Critical Reviews in Clinical Laboratory Sciences. 58 (6): 385–398. doi:10.1080/10408363.2021.1884640. ISSN 1549-781X. PMC 7898297. PMID 33595397.
^ Kurosaki, Yohei; Magassouba, N’Faly; Oloniniyi, Olamide K.; Cherif, Mahamoud S.; Sakabe, Saori; Takada, Ayato; Hirayama, Kenji; Yasuda, Jiro (2016-02-22). "Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea". PLOS Neglected Tropical Diseases. 10 (2): e0004472. doi:10.1371/journal.pntd.0004472. ISSN 1935-2735. PMC 4764121. PMID 26900929.

[Mori_2009-1] Mori Y, Notomi T (2009). "Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases". J. Infect. Chemother. 15 (2): 62–9. doi:10.1007/s10156-009-0669-9. PMC 7087713. PMID 19396514.

[2] Notomi, Tsugunori; Okayama, Hiroto; Masubuchi, Harumi; Yonekawa, Toshihiro; Watanabe, Keiko; Amino, Nobuyuki; Hase, Tetsu (2000-06-15). "Loop-mediated isothermal amplification of DNA". Nucleic Acids Research. 28 (12): e63. doi:10.1093/nar/28.12.e63. ISSN 0305-1048. PMC 102748. PMID 10871386.

[Fu_2011-3] Fu S, Qu G, Guo S, Ma L, Zhang N, Zhang S, Gao S, Shen Z (2011). "Applications of loop-mediated isothermal DNA amplification". Appl. Biochem. Biotechnol. 163 (7): 845–50. doi:10.1007/s12010-010-9088-8. PMID 20844984. S2CID 45682156.

[4] Habibzadeh, Parham; Mofatteh, Mohammad; Silawi, Mohammad; Ghavami, Saeid; Faghihi, Mohammad Ali (2021-02-17). "Molecular diagnostic assays for COVID-19: an overview". Critical Reviews in Clinical Laboratory Sciences. 58 (6): 385–398. doi:10.1080/10408363.2021.1884640. ISSN 1549-781X. PMC 7898297. PMID 33595397.

[5] Kurosaki, Yohei; Magassouba, N’Faly; Oloniniyi, Olamide K.; Cherif, Mahamoud S.; Sakabe, Saori; Takada, Ayato; Hirayama, Kenji; Yasuda, Jiro (2016-02-22). "Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea". PLOS Neglected Tropical Diseases. 10 (2): e0004472. doi:10.1371/journal.pntd.0004472. ISSN 1935-2735. PMC 4764121. PMID 26900929.

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