Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact tissue.[1] The historical precursor to spatial transcriptomics is in situ hybridization,[2] where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets. It comprises an important part of spatial biology.
Spatial transcriptomics includes methods that can be divided into two modalities, those based in next-generation sequencing for gene detection, and those based in imaging.[3] Some common approaches to resolve spatial distribution of transcripts are microdissection techniques, fluorescent in situ hybridization methods, in situ sequencing, in situ capture protocols and in silico approaches.[4]
- ^ "Method of the Year 2020: spatially resolved transcriptomics". Nature Methods. 18 (1): 1. January 2021. doi:10.1038/s41592-020-01042-x. ISSN 1548-7105. PMID 33408396.
- ^ Gall JG (April 2016). "The origin of in situ hybridization – A personal history". Methods. 98: 4–9. doi:10.1016/j.ymeth.2015.11.026. ISSN 1046-2023. PMC 4808352. PMID 26655524.
- ^ Tian L, Chen F, Macosko EZ (June 2023). "The expanding vistas of spatial transcriptomics". Nature Biotechnology. 41 (6): 773–782. doi:10.1038/s41587-022-01448-2. PMC 10091579. PMID 36192637.
- ^ Asp M, Bergenstråhle J, Lundeberg J (October 2020). "Spatially Resolved Transcriptomes-Next Generation Tools for Tissue Exploration". BioEssays. 42 (10): e1900221. doi:10.1002/bies.201900221. PMID 32363691. S2CID 218492475.
© MMXXIII Rich X Search. We shall prevail. All rights reserved. Rich X Search