Two-dimensional gel electrophoresis

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. 2-DE was first independently introduced by O'Farrell^[1] and Klose^[2] in 1975.

^ O'Farrell, PH (1975). "High resolution two-dimensional electrophoresis of proteins". J. Biol. Chem. 250 (10): 4007–21. doi:10.1016/S0021-9258(19)41496-8. PMC 2874754. PMID 236308.
^ Klose, J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals". Humangenetik. 26 (3): 231–43. doi:10.1007/bf00281458. PMID 1093965. S2CID 30981877.

[1] O'Farrell, PH (1975). "High resolution two-dimensional electrophoresis of proteins". J. Biol. Chem. 250 (10): 4007–21. doi:10.1016/S0021-9258(19)41496-8. PMC 2874754. PMID 236308.

[2] Klose, J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals". Humangenetik. 26 (3): 231–43. doi:10.1007/bf00281458. PMID 1093965. S2CID 30981877.

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